Composite

Part:BBa_K2680068

Designed by: Stephanie Do, Xiangyi Fang, Ethan Jones, Jessica Laury, Adam Oliver, Lillian Parr, Chengwu Shen, Tinh Son, Julia Urban, Yashna Verma, Hanmi Zou   Group: iGEM18_William_and_Mary   (2018-10-06)


J23107 ts-CI + R0051 mScarlet-I

37C Temperature Sensitive mScarlet-I

Temperature-Sensitive mScarlet-I. Components: J23107 ts-CI + R0051 mScarlet-I

Mechanics

In this circuit, temperature-sensitive CI is expressed constitutionally by J23107, a medium-weak constitutive promoter. This temperature-sensitive CI then represses the downstream CI promoter. Once the temperature is raised to approximately 37, the ts-CI protein denatures, losing its repression capabilities. Therefore, the CI promoter is freed, allowing for expression of the rapidly-maturing mScarlet-I.

Role in an IFFL System

While this circuit is not an IFFL, it acts as a control to determine the normal rate of expression of mScarlet-I given interaction between the ts-CI and the CI promoter as temperature changes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 51
    Illegal NheI site found at 74
    Illegal NotI site found at 1518
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None